Mfge8^tm1Shur
Targeted Allele Detail

Summary

• Symbol: Mfge8^tm1Shur
• Name: milk fat globule EGF and factor V/VIII domain containing; targeted mutation 1, Barry D Shur
• MGI ID: MGI:2675967
• Synonyms: SED1-null
• Gene: Mfge8 Location: Chr7:78783516-78798808 bp, - strand Genetic Position: Chr7, 44.9 cM
• Alliance: Mfge8^tm1Shur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85225
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxP site remained in place of exon 1 after homologous recombination of flanking loxP sites and subsequent cre-mediated excision. Transcript was undetected in homozygous mutant mice by RT-PCR analysis of testicular cDNA using primers spanning exons 2 and 3 and exons 7 and 8. The absence of protein was indicated by Western blot analysis and by indirect immunofluorescence of live sperm. (J:85225)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfge8 Mutation: 23 strains or lines available

References

• Original: J:85225 Ensslin MA, et al., Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg binding. Cell. 2003 Aug 22;114(4):405-17
• All: 6 reference(s)