Fbxo32^tm1Glas
Targeted Allele Detail

Summary

• Symbol: Fbxo32^tm1Glas
• Name: F-box protein 32; targeted mutation 1, David J Glass
• MGI ID: MGI:2676047
• Synonyms: MAFbx -
• Gene: Fbxo32 Location: Chr15:58039275-58078288 bp, - strand Genetic Position: Chr15, 24.43 cM, cytoband D2
• Alliance: Fbxo32^tm1Glas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77660
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An in-frame lacZ gene and neomycin selection cassette replaced approximately 35 kb of gene sequences, including exons 1-7 and most of exon 8. Northern blot analysis on RNA derived from muscle of homozygous mice confirmed that no wild-type transcript is expressed from this allele. Beta-galactosidase is expressed from this allele under the control of the endogenous promoter. (J:77660)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Fbxo32^tm1Glas/Fbxo32^tm1Glas	involves: 129S1/Sv

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
muscle	√
decreased susceptibility to induced muscular atrophy	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxo32 Mutation: 17 strains or lines available

References

• Original: J:77660 Bodine SC, et al., Identification of ubiquitin ligases required for skeletal muscle atrophy. Science. 2001 Nov 23;294(5547):1704-8
• All: 8 reference(s)