Lgmn^tm1Ihn
Targeted Allele Detail

Summary

• Symbol: Lgmn^tm1Ihn
• Name: legumain; targeted mutation 1, Ikuko Hara-Nishimura
• MGI ID: MGI:2676125
• Synonyms: AEP^-
• Gene: Lgmn Location: Chr12:102360341-102405987 bp, - strand Genetic Position: Chr12, 51.45 cM
• Alliance: Lgmn^tm1Ihn page

Enlargement of lysosomes in the kidney-proximal tubule cells of Lgmn^tm1Ihn/Lgmn^tm1Ihn mice

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85207
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 3 exons were flanked by loxP sites and excised prior to germline transmission via cre-mediated recombination. An frt-flanked neo cassette was left in an intron. Neither transcript nor protein was detected in homozygous mutant mice by RT-PCR and Western blot analyses of kidney tissue. (J:85207)

Disease models

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgmn Mutation: 35 strains or lines available

References

• Original: J:85207 Shirahama-Noda K, et al., Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice. J Biol Chem. 2003 Aug 29;278(35):33194-9
• All: 22 reference(s)