Thrb^tm3Few
Targeted Allele Detail

Summary

• Symbol: Thrb^tm3Few
• Name: thyroid hormone receptor beta; targeted mutation 3, Frederic E Wondisford
• MGI ID: MGI:2676923
• Synonyms: GS125 KI, TR-Beta^GS
• Gene: Thrb Location: Chr14:4431611-4809435 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Thrb^tm3Few page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85158
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Glutamic acid codon 125 (GAA) in exon 3 was changed to glycine (GGA) (p.E125G) and glycine codon 126 (GGC) to serine (AGC) (p.G126S). The mutations are within the P-box of the first zinc finger of the encoded peptide and were shown, in vitro, to abolish DNA-binding while retaining the ability to interact with T3 and cofactors. A self-excising ACN cassette (loxP site + testis-specific ACE promoter driven cre gene + neomycin resistance gene cassette + loxP site) was inserted into intron 3. Western blot analysis showed endogenous levels of protein in homozygous mutant mice. (J:85158)

Disease models

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thrb Mutation: 41 strains or lines available

References

• Original: J:85158 Shibusawa N, et al., Thyroid hormone action in the absence of thyroid hormone receptor DNA-binding in vivo. J Clin Invest. 2003 Aug;112(4):588-97
• All: 4 reference(s)