Airn^tm1Dpb
Targeted Allele Detail

Summary

• Symbol: Airn^tm1Dpb
• Name: antisense Igf2r RNA; targeted mutation 1, Denise P Barlow
• MGI ID: MGI:2677157
• Synonyms: Air-T, Air^tm1Dpb-pat
• Gene: Airn Location: Chr17:12960198-13078771 bp, + strand Genetic Position: Chr17, 8.66 cM
• Alliance: Airn^tm1Dpb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77594
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A 1.2 kb rabbit beta-globin fragment containing a polyadenylation cassette was inserted immediately downstream of the 3.7 kb imprint control element (ICE). The insertion was designed to truncate the RNA product to 4% of its length without disrupting the ICE. While the allele maintains its imprinted status and wild-type expression levels, the truncation results in a loss of gene silencing of the Igf2r/Slc22a2/Slc22a3 cluster on the paternal chromosome. An aberrant transcript was identified by RT-PCR analysis. This splice varient involved a splice donor 53 bp downstream of the transcription start site that joined a slice acceptor of transgenic beta-globin exon 3. (J:77594)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Airn Mutation: 4 strains or lines available

References

• Original: J:77594 Sleutels F, et al., The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature. 2002 Feb 14;415(6873):810-3
• All: 3 reference(s)