Sirt1^tm2.1Fwa
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm2.1Fwa
• Name: sirtuin 1; targeted mutation 2.1, Frederick W Alt
• MGI ID: MGI:2677369
• Synonyms: SIRT1^-, SIRT1^delta, SIRT1^null, SIRT null
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm2.1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85542
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxP site remained in place of exons 5, 6, and 7 after the floxed neo cassette inserted by homologous recombination was excised via in vitro cre-mediated recombination. Protein was undetected in homozygous mutant MEFs by Western blot analysis using an antibody specific for the N-terminal. (J:85542)

Expression

In Mice Carrying this Mutation:	2 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:85542 Cheng HL, et al., Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9
• All: 31 reference(s)