Nkx2-1^tm1Jaw
Targeted Allele Detail

Summary

• Symbol: Nkx2-1^tm1Jaw
• Name: NK2 homeobox 1; targeted mutation 1, Jeffrey A Whitsett
• MGI ID: MGI:2677488
• Synonyms: Titf1^PM
• Gene: Nkx2-1 Location: Chr12:56578741-56583570 bp, - strand Genetic Position: Chr12, 24.42 cM, cytoband C1-C3
• Alliance: Nkx2-1^tm1Jaw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85424
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding S80, a phosphorylation mutant allele of the orthologous rat gene in which 7 serine codons are replaced with alanine codons, was inserted in place of a fragment extending from the start codon (bp 7957) to the end of the homeobox (bp 9480). A polyadenylation signal and neo cassette were included immediately downstream of S80. Southern blot analysis confirmed the integration of the mutation that putatively produces a protein lacking residues required for phosphorylation. (J:85424)

Expression

In Mice Carrying this Mutation:	41 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-1 Mutation: 24 strains or lines available

Notes

ES cell line = MPI1 (MPI-I).

References

• Original: J:85424 DeFelice M, et al., TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression. J Biol Chem. 2003 Sep 12;278(37):35574-83
• All: 3 reference(s)