Hba-a1^tm1Led
Targeted Allele Detail

Summary

• Symbol: Hba-a1^tm1Led
• Name: hemoglobin alpha, adult chain 1; targeted mutation 1, Philip Leder
• MGI ID: MGI:2677498
• Synonyms: alpha1-
• Gene: Hba-a1 Location: Chr11:32233672-32234486 bp, + strand Genetic Position: Chr11, 18.86 cM
• Alliance: Hba-a1^tm1Led page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41934
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a small 295 bp fragment in the second exon of the gene. Northern blot analysis on RNA derived from homozygous embryos demonstrated that a reduced amount of alpha-globin transcript was present in comparison to wild type. The authors propose that the remaining mRNA arose from the adjacent Hba-a2 gene. (J:41934)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hba-a1 Mutation: 3 strains or lines available

References

• Original: J:41934 Leder A, et al., Mouse zeta- and alpha-globin genes: Embryonic survival, alpha-thalassemia, and genetic background effects. Blood. 1997 AUG 1;90(3):1275-1282
• All: 2 reference(s)