Sftpd^tm2Haw
Targeted Allele Detail

Summary

• Symbol: Sftpd^tm2Haw
• Name: surfactant associated protein D; targeted mutation 2, Samuel Hawgood
• MGI ID: MGI:2677969
• Synonyms: SP-AD(-)
• Gene: Sftpd Location: Chr14:40894171-40907106 bp, - strand Genetic Position: Chr14, 22.36 cM
• Alliance: Sftpd^tm2Haw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86022
• Parent Cell Line: CB1-4 (ES Cell)
• Strain of Origin: C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using ES cells in which a correctly targeted modification of the Sftpa gene had already been created, exon 2 along with the translation start site, was replaced with a PGK-neomycin cassette. Northern blot analysis demonstrated the absence of any Sftpa mRNA in any doubly homozygous mice as well as the absence of full length Sftpd mRNA. A small amount of truncated mRNA for Sftpd lacking the translation start site was seen. Western blot analysis confirmed that no protein products were produced. (J:86022)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sftpd Mutation: 17 strains or lines available

Notes

This allele was generated in ES cells containing Sftpa1^tm2Haw.

References

• Original: J:86022 Hawgood S, et al., Sequential targeted deficiency of SP-A and -D leads to progressive alveolar lipoproteinosis and emphysema. Am J Physiol Lung Cell Mol Physiol. 2002 Nov;283(5):L1002-10
• All: 5 reference(s)