Klk8^tm1Shio
Targeted Allele Detail

Summary

• Symbol: Klk8^tm1Shio
• Name: kallikrein related-peptidase 8; targeted mutation 1, Sadao Shiosaka
• MGI ID: MGI:2678312
• Synonyms: Neuropsin^-, NP^-
• Gene: Klk8 Location: Chr7:43447001-43453250 bp, + strand Genetic Position: Chr7, 28.26 cM, cytoband B4
• Alliance: Klk8^tm1Shio page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68653
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 through 3 were replaced with a neomycin selection cassette. The deleted region encoded histidine at postion 57 and aspartate 102 which are essential for catalytic activity. Transcript was undetected by Northern blot analysis of homozygous mutant mice. Neuropsin activity was undetected in immunoprecipitate from hippocampal homogenate obtained from homozygous mutant mice. (J:68653)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Klk8 Mutation: 22 strains or lines available

References

• Original: J:68653 Hirata A, et al., Abnormalities of synapses and neurons in the hippocampus of neuropsin-deficient mice. Mol Cell Neurosci. 2001 Mar;17(3):600-10
• All: 12 reference(s)