Top1^tm1Unc
Targeted Allele Detail

Summary

• Symbol: Top1^tm1Unc
• Name: topoisomerase (DNA) I; targeted mutation 1, University of North Carolina
• MGI ID: MGI:2678314
• Synonyms: Top-1 -
• Gene: Top1 Location: Chr2:160487901-160564684 bp, + strand Genetic Position: Chr2, 80.96 cM, cytoband H2-3
• Alliance: Top1^tm1Unc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36546
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing exon 15. Western blot analysis demonstrated that a 45% reduction of encoded protein levels was present in cells derived from heterozygous mice. Cytological analysis of early homozygous mutant embryos demonstrated that no detectable protein was produced from this allele. (J:36546)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Top1 Mutation: 210 strains or lines available

References

• Original: J:36546 Morham SG, et al., Targeted disruption of the mouse topoisomerase I gene by camptothecin selection. Mol Cell Biol. 1996 Dec;16(12):6804-9
• All: 1 reference(s)