Itsn1^{Gt(pT1ATGBetageo)1Ruiz}
Gene trapped Allele Detail

Summary

• Symbol: Itsn1^{Gt(pT1ATGBetageo)1Ruiz}
• Name: intersectin 1 (SH3 domain protein 1A); gene trap 1, Patricia Ruiz
• MGI ID: MGI:2678392
• Synonyms: Itsn1^Gt
• Gene: Itsn1 Location: Chr16:91526198-91717479 bp, + strand Genetic Position: Chr16, 53.28 cM, cytoband C4
• Alliance: Itsn1^{Gt(pT1ATGBetageo)1Ruiz} page

Mutation origin

• Mutant Cell Line: F053A01
• Germline Transmission: Earliest citation of germline transmission: J:85162
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pT1ATGbetageo
• Mutation details: The endogenous locus was disrupted by the insertion of a gene trap vector containing a splice acceptor site followed by Beta-geo and a polyadenylation signal. (J:85162)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  F053A01	CL211077 ; (MGI Seq Detail)	5' RACE	unknown
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itsn1 Mutation: 101 strains or lines available

Notes

Information submitted to the NCBI Genome Survey Sequences Database (dbGSS) by the German Genetrap Consortium (GGTC) for gene trap cell line F053A01 gives R1 as the ES cell line used to create this allele. The original publication reporting large scale production of gene trap clones using four gene trap vectors says that ES cell-derived chimeras were produced using three ES cell lines (E14.1 (129P2/OlaHsd), R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+), TBV2 (129S2/SvPas)). GGTC records currently say that TBV2 ES cells were used to generate this allele. Chimeric mice were subsquently bred to C57BL/6 females.

References

• Original: J:85162 Hansen J, et al., A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9918-22
• All: 3 reference(s)