Plec^{Gt(U3Betageo)1Ruiz}
Gene trapped Allele Detail

Summary

• Symbol: Plec^{Gt(U3Betageo)1Ruiz}
• Name: plectin; gene trap 1, Patricia Ruiz
• MGI ID: MGI:2678403
• Gene: Plec Location: Chr15:76055174-76115578 bp, - strand Genetic Position: Chr15, 35.48 cM
• Alliance: Plec^{Gt(U3Betageo)1Ruiz} page

Mutation origin

• Mutant Cell Line: M020A01
• Germline Transmission: Earliest citation of germline transmission: J:85162
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: U3betageo
• Mutation details: The endogenous locus was disrupted by the insertion of retroviral gene trap vector containing Beta-geo. The vector lacked a splice acceptor and therefore putatively inserted within an exon. (J:85162)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  M020A01	CL211465 ; (MGI Seq Detail)	5' RACE	Chr15:76069853-76070396 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plec Mutation: 174 strains or lines available

Notes

Information submitted to the NCBI Genome Survey Sequences Database (dbGSS) by the German Genetrap Consortium (GGTC) for gene trap cell line M020A01 gives TBV2 as the ES cell line used to create this allele. The original publication reporting large scale production of gene trap clones using four gene trap vectors says that ES cell-derived chimeras were produced using three ES cell lines (E14.1 (129P2/OlaHsd), R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl⁺), TBV2 (129S2/SvPas)). GGTC records currently say that TBV2 ES cells were used to generate this allele. Chimeric mice were subsquently bred to C57BL/6 females.

References

• Original: J:85162 Hansen J, et al., A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9918-22
• All: 1 reference(s)