Opn1mw^{tm1(OPN1LW)Nat}
Targeted Allele Detail

Summary

• Symbol: Opn1mw^{tm1(OPN1LW)Nat}
• Name: opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan); targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:2678763
• Synonyms: Opn1mw^R, Opn1mw^red, R
• Gene: Opn1mw Location: ChrX:73171072-73194362 bp, + strand Genetic Position: ChrX, Syntenic
• Alliance: Opn1mw^{tm1(OPN1LW)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85849
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Opn1mw^{tm1(OPN1LW)Nat} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  OPN1LW (5956)

  Opn1mw

  (MGI:1097692)

• Opn1mw^{tm1(OPN1LW)Nat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Opn1mw (MGI:1097692)

• Mutation details: Human cDNA encoding OPN1LW was inserted at the endogenous mouse Opn1mw locus, replacing a 3' portion of exon 2 and all of exons 3, 4, and 5. The allele putatively produces a hybrid protein consisting of 92 N-terminal residues encoded by mouse exon 1 and the 5' portion of exon 2, and 266 C-terminal residues encoded by the human cDNA. Nearly the entire chromophore binding pocket, as well as the residues that account for the difference in absorbance spectra between human and mouse, is derived from the human sequence. Immunostaining indicated expression of the hybrid protein and electroretinogram indicated that the spectral sensitivity of cones containing the targeted allele was similar to that of human red cones. (J:85849)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Opn1mw Mutation: 5 strains or lines available

References

• Original: J:85849 Smallwood PM, et al., Genetically engineered mice with an additional class of cone photoreceptors: implications for the evolution of color vision. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11706-11
• All: 18 reference(s)