Crebbp^tm1Reck
Targeted Allele Detail

Summary

• Symbol: Crebbp^tm1Reck
• Name: CREB binding protein; targeted mutation 1, Richard Eckner
• MGI ID: MGI:2679376
• Synonyms: cbp^AS
• Gene: Crebbp Location: Chr16:3899198-4031864 bp, - strand Genetic Position: Chr16, 2.4 cM
• Alliance: Crebbp^tm1Reck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85950
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Dominant negative, Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: Acetyltransferase (AT) activity was ablated via the incorporation of engineered point mutations at the endogenous locus. The mutations substitute alanine and serine for tryptophan and tyrosine (WY(1503-1504)AS). A single loxP site remained in the intron preceding the missense mutations after the excision of floxed neo cassette used for selection. Western blot analysis indicated mutant protein was expressed at wild-type levels. (J:85950)
• Inheritance: Dominant

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crebbp Mutation: 100 strains or lines available

References

• Original: J:85950 Roth JF, et al., Differential role of p300 and CBP acetyltransferase during myogenesis: p300 acts upstream of MyoD and Myf5. EMBO J. 2003 Oct 1;22(19):5186-96
• All: 2 reference(s)