Eif2ak1^tm1Jjch
Targeted Allele Detail

Summary

• Symbol: Eif2ak1^tm1Jjch
• Name: eukaryotic translation initiation factor 2 alpha kinase 1; targeted mutation 1, Jane-Jane Chen
• MGI ID: MGI:2679454
• Synonyms: HRI^-
• Gene: Eif2ak1 Location: Chr5:143803586-143839535 bp, + strand Genetic Position: Chr5, 82.67 cM
• Alliance: Eif2ak1^tm1Jjch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85896
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Three exons containing kinase domains VIb - X were replaced with a floxed PGK neomycin gene. The PGK neomicin gene was then removed by cre excision from mice heterozygous for this allele. mRNA transcripts were 404 bp shorter than wild-type. No protein product was detectable by Western blot. (J:85896)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eif2ak1 Mutation: 37 strains or lines available

References

• Original: J:85896 Han AP, et al., Heme-regulated eIF2alpha kinase (HRI) is required for translational regulation and survival of erythroid precursors in iron deficiency. EMBO J. 2001 Dec 3;20(23):6909-18
• All: 14 reference(s)