Acacb^tm1Sjw
Targeted Allele Detail

Summary

• Symbol: Acacb^tm1Sjw
• Name: acetyl-Coenzyme A carboxylase beta; targeted mutation 1, Salih J Wakil
• MGI ID: MGI:2679547
• Synonyms: Acc2^-, Acc2^tm1AE
• Gene: Acacb Location: Chr5:114284748-114388822 bp, + strand Genetic Position: Chr5, 55.97 cM
• Alliance: Acacb^tm1Sjw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68427
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The exon encoding the biotin binding motif and flanking genomic sequence was replaced by an HPRT expression cassette by homologous recombination. Northern blot analysis on RNA derived from skeletal muscle demonstrated that no detectable transcript was expressed from this allele in homozygous mice. Western blot analysis on extracts of various tissues confirmed that no detectable protein was present in homozygous mice. (J:68427)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acacb Mutation: 109 strains or lines available

References

• Original: J:68427 Abu-Elheiga L, et al., Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2. Science. 2001 Mar 30;291(5513):2613-6
• All: 9 reference(s)