Slc19a1^tm1Gold
Targeted Allele Detail

Summary

• Symbol: Slc19a1^tm1Gold
• Name: solute carrier family 19 (folate transporter), member 1; targeted mutation 1, I David Goldman
• MGI ID: MGI:2679677
• Synonyms: RFC1^-
• Gene: Slc19a1 Location: Chr10:76868103-76886266 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Slc19a1^tm1Gold page

Slc19a1^tm1Gold/Slc19a1^tm1Gold embryos are small and deformed at E9.5

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68666
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 3' portion of exon 3, which contains the start codon, was replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant MEFs using a C terminal antibody. (J:68666)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc19a1 Mutation: 31 strains or lines available

References

• Original: J:68666 Zhao R, et al., Rescue of embryonic lethality in reduced folate carrier-deficient mice by maternal folic acid supplementation reveals early neonatal failure of hematopoietic organs. J Biol Chem. 2001 Mar 30;276(13):10224-8
• All: 2 reference(s)