Sh2b2^tm1Yeb
Targeted Allele Detail

Summary

• Symbol: Sh2b2^tm1Yeb
• Name: SH2B adaptor protein 2; targeted mutation 1, Yousuke Ebina
• MGI ID: MGI:2680256
• Synonyms: APS^-
• Gene: Sh2b2 Location: Chr5:136247001-136275410 bp, - strand Genetic Position: Chr5, 75.93 cM, cytoband G1
• Alliance: Sh2b2^tm1Yeb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86301
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: Exons 1 through 6 were replaced with a neomycin selection cassette. Normal transcript was undetected by Northern blot analysis of homozygous mutant skeletal muscle and adipose tissue. While truncated transcript was identified in adipose tissue, protein was undetected by Western blot analysis using antibodies directed toward the N- and C-terminals. (J:86301)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sh2b2 Mutation: 37 strains or lines available

References

• Original: J:86301 Minami A, et al., Increased insulin sensitivity and hypoinsulinemia in APS knockout mice. Diabetes. 2003 Nov;52(11):2657-65
• All: 4 reference(s)