Mapkapk5^tm1Mgl
Targeted Allele Detail

Summary

• Symbol: Mapkapk5^tm1Mgl
• Name: MAP kinase-activated protein kinase 5; targeted mutation 1, Matthias Gaestel
• MGI ID: MGI:2680287
• Synonyms: deltaex6, MK5-
• Gene: Mapkapk5 Location: Chr5:121663114-121683955 bp, - strand Genetic Position: Chr5, 61.84 cM, cytoband F
• Alliance: Mapkapk5^tm1Mgl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86268
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 6 was replaced with a neo cassette. The targeted exon encoded amino acids 131 through 161 which make up portions of the kinase catalytic core subdomains VIa and VIb. Northern blot and RT-PCR analyses identified mutant transcript approximately 100 bp shorter than normal transcript. Sequence analysis showed the mutant transcript to contain neither exon 6 nor the neo cassette. Low levels of truncated protein were detected by Western blot analysis, but this protein was reported to lack catalytic activity. (J:86268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapkapk5 Mutation: 30 strains or lines available

References

• Original: J:86268 Shi Y, et al., Elimination of protein kinase MK5/PRAK activity by targeted homologous recombination. Mol Cell Biol. 2003 Nov;23(21):7732-41
• All: 5 reference(s)