Itpkb^tm1Ssch
Targeted Allele Detail

Summary

• Symbol: Itpkb^tm1Ssch
• Name: inositol 1,4,5-trisphosphate 3-kinase B; targeted mutation 1, Stephane Schurmans
• MGI ID: MGI:2680720
• Synonyms: Itpkb^-
• Gene: Itpkb Location: Chr1:180158050-180252367 bp, + strand Genetic Position: Chr1, 84.27 cM, cytoband H5
• Alliance: Itpkb^tm1Ssch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86257
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 0.3 kb of the first coding exon was replaced with a neomycin selection cassette inserted by homologous recombination. Transcript containing the neo cassette was detected by RT-PCR analysis of mutant mice. Mutant protein potentially encoded by the detected transcript lacks the catalytic unit and is putatively nonfunctional. Inositol 1,4,5-triphosphate 3-kinase activity was attenuated in a dose dependent manner in mutant mice. (J:86257)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itpkb Mutation: 40 strains or lines available

References

• Original: J:86257 Pouillon V, et al., Inositol 1,3,4,5-tetrakisphosphate is essential for T lymphocyte development. Nat Immunol. 2003 Nov;4(11):1136-43
• All: 9 reference(s)