Il4ra^tm1Tch
Targeted Allele Detail

Summary

• Symbol: Il4ra^tm1Tch
• Name: interleukin 4 receptor, alpha; targeted mutation 1, Talal A Chatila
• MGI ID: MGI:2680722
• Synonyms: Y500F
• Gene: Il4ra Location: Chr7:125151443-125178646 bp, + strand Genetic Position: Chr7, 68.94 cM
• Alliance: Il4ra^tm1Tch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86215
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A single A to T substitution was created at the position equivalent to 1735 of the cDNA. This caused a codon change for amino acid residue 500 from tyrosine to phenylalanine (Y500F) disrupting the PTB domain. In addition, a floxed PGK-neomycin resistance cassette was inserted into intron 11. This cassette was removed from successfully targeted clones by transient transfection with a Cre recombinase. The mutant is transcribed at rates equivalent to those of wild-type as indicated by RT-PCR. (J:86215)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il4ra Mutation: 46 strains or lines available

References

• Original: J:86215 Blaeser F, et al., Targeted inactivation of the IL-4 receptor alpha chain I4R motif promotes allergic airway inflammation. J Exp Med. 2003 Oct 20;198(8):1189-200
• All: 3 reference(s)