Capg^tm1Djk
Targeted Allele Detail

Summary

• Symbol: Capg^tm1Djk
• Name: capping actin protein, gelsolin like; targeted mutation 1, David J Kwiatkowski
• MGI ID: MGI:2680796
• Synonyms: Capg^-
• Gene: Capg Location: Chr6:72521374-72539966 bp, + strand Genetic Position: Chr6, 32.27 cM, cytoband C3
• Alliance: Capg^tm1Djk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86517
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 7 and 8 were replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected in homozygous mutant mice by Western blot analysis of various tissues. Further analysis of mutant bone marrow-derived macrophages using an antibody directed toward the N-terminal region confirmed the absence of truncated protein. (J:86517)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Capg Mutation: 18 strains or lines available

References

• Original: J:86517 Witke W, et al., Comparisons of CapG and gelsolin-null macrophages: demonstration of a unique role for CapG in receptor-mediated ruffling, phagocytosis, and vesicle rocketing. J Cell Biol. 2001 Aug 20;154(4):775-84
• All: 2 reference(s)