Msh2^tm1Wed
Targeted Allele Detail

Summary

• Symbol: Msh2^tm1Wed
• Name: mutS homolog 2; targeted mutation 1, Winfried Edelmann
• MGI ID: MGI:2680817
• Synonyms: Msh2^G674A, Msh2^tm1Mtn
• Gene: Msh2 Location: Chr17:87979960-88031141 bp, + strand Genetic Position: Chr17, 57.87 cM
• Alliance: Msh2^tm1Wed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86278
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The incorporation of an engineered point mutation in exon 13 created a glycine to alanine substitution at codon 674 (G674A), within the conserved ATPase domain of the C-terminal. A floxed neo-PGK-hygro cassette that was included in the targeting vector was removed by cre-mediated recombination leaving a single loxP site and the G674A mutation. Western blot analysis of homozygous mutant MEFs indicated expression of the mutant protein at wild-type levels. (J:88092)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Msh2 Mutation: 95 strains or lines available

References

• Original: J:86278 Martin A, et al., Msh2 ATPase activity is essential for somatic hypermutation at a-T basepairs and for efficient class switch recombination. J Exp Med. 2003 Oct 20;198(8):1171-8
• All: 4 reference(s)