Rho^tm1Jlem
Targeted Allele Detail

Summary

• Symbol: Rho^tm1Jlem
• Name: rhodopsin; targeted mutation 1, Janis Lem
• MGI ID: MGI:2680822
• Synonyms: mrho^-, Opsin -, rhodopsin-
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^tm1Jlem page

Retinal morphology of Rho^tm1Jlem/Rho⁺ and Rho^tm1Jlem/Rho^tm1Jlem mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52281
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette was inserted into the first coding exon of the endogenous gene, deleting 15 bp upstream of the translational start site and 111 codons. Transcript was undetected in homozygous mutant mice by Northern blot and RT-PCR analyses. Western blot analysis indicated an absence of protein in whole retinal homogenates. (J:52281)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 51 strains or lines available

References

• Original: J:52281 Lem J, et al., Morphological, physiological, and biochemical changes in rhodopsin knockout mice. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):736-41
• All: 52 reference(s)