Dicer1^tm1Ghan
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm1Ghan
• Name: dicer 1, ribonuclease type III; targeted mutation 1, Gregory J Hannon
• MGI ID: MGI:2681012
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm1Ghan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86391
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassete replaced exon 23 (previously called exon 21), which encodes the second RNase III domain. The splice donor and acceptor sites were left intact to further the potential of a null allele by permitting the incorporation of the neo cassette, and thus stop codons in all three reading frames, into the message. (J:86391)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:86391 Bernstein E, et al., Dicer is essential for mouse development. Nat Genet. 2003 Nov;35(3):215-7
• All: 6 reference(s)