Pnlip^tm1Dyh
Targeted Allele Detail

Summary

• Symbol: Pnlip^tm1Dyh
• Name: pancreatic lipase; targeted mutation 1, David Y Hui
• MGI ID: MGI:2681122
• Synonyms: PTL^-
• Gene: Pnlip Location: Chr19:58658797-58670231 bp, + strand Genetic Position: Chr19, 54.46 cM, cytoband D3
• Alliance: Pnlip^tm1Dyh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86414
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 4 was disrupted by a neomycin selection cassette inserted by homologous recombination. The targeted exon has been reported to be essential for enzymatic activity. Protein was undetected in homozygous mutant mice by Western blot analysis of pancreatic extracts. Triglyceride hydrolysis was reduced by approximately 90% in homozygous mutanat mice. (J:86414)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pnlip Mutation: 28 strains or lines available

References

• Original: J:86414 Huggins KW, et al., Pancreatic triglyceride lipase deficiency minimally affects dietary fat absorption but dramatically decreases dietary cholesterol absorption in mice. J Biol Chem. 2003 Oct 31;278(44):42899-905
• All: 4 reference(s)