Igh-J^{tm1(VDJ-KL25)Zbz}
Targeted Allele Detail

Summary

• Symbol: Igh-J^{tm1(VDJ-KL25)Zbz}
• Name: immunoglobulin heavy chain, joining region; targeted mutation 1, Universitatsspital Zurich, Biologisches Zentrallaboratorium
• MGI ID: MGI:2681436
• Synonyms: TgH(KL25)
• Gene: Igh-J Location: unknown Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86411
• Parent Cell Line: BL/6-III (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous DQ52 and all four heavy chain J elements were replaced by the rearranged heavy chain VDJ region of hybridoma KL25 5.8 (for a LCMV-neutralizing antibody) and a floxed neomycin resistance cassette. The neomycin cassette was removed in vivo by breeding to Cre deleter mice. This allele is expressed in more than 95% of all B cells. (J:86411)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
ht1	Igh-J^tm1(VDJ-KL25)Zbz/Igh-J^+	involves: C57BL/6 * C57BL/6J * BALB/cJ

Phenotypes:

Affected Systems	ht1
show or hide all annotated terms
hematopoietic system	√
increased IgG level	√
increased IgM level	√
immune system	√
increased IgG level	√
increased IgM level	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-J Mutation: 13 strains or lines available

References

• Original: J:86411 Hangartner L, et al., Antiviral immune responses in gene-targeted mice expressing the immunoglobulin heavy chain of virus-neutralizing antibodies. Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12883-8
• All: 11 reference(s)