Gcnt1^tm1Jxm
Targeted Allele Detail

Summary

• Symbol: Gcnt1^tm1Jxm
• Name: glucosaminyl (N-acetyl) transferase 1, core 2; targeted mutation 1, Jamey Marth
• MGI ID: MGI:2681554
• Synonyms: C2GlcNAcT^delta, C2GlcNAcT-I^-, C2GlcNAcT-I^null, C2GnT1^-, Core2GlcNAcT
• Gene: Gcnt1 Location: Chr19:17303505-17350208 bp, - strand Genetic Position: Chr19, 12.75 cM
• Alliance: Gcnt1^tm1Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86387
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The single protein coding exon was flanked upstream by a loxP site and downstream by a floxed neomycin resistance cassette. Transient Cre expression in correctly targeted cells resulted in removal of both the coding exon and the neomycin cassette. Enzyme activity was absent in spleen, bone marrow, and kidney tissue of homozygotes. (J:86387)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gcnt1 Mutation: 30 strains or lines available

References

• Original: J:86387 Ellies LG, et al., Core 2 oligosaccharide biosynthesis distinguishes between selectin ligands essential for leukocyte homing and inflammation. Immunity. 1998 Dec;9(6):881-90
• All: 31 reference(s)