Bbc3^tm1Ast
Targeted Allele Detail

Summary

• Symbol: Bbc3^tm1Ast
• Name: BCL2 binding component 3; targeted mutation 1, Andreas Strasser
• MGI ID: MGI:2681654
• Synonyms: puma^-
• Gene: Bbc3 Location: Chr7:16042318-16052130 bp, + strand Genetic Position: Chr7, 8.85 cM
• Alliance: Bbc3^tm1Ast page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86466
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 which contain the BH3 interaction domain were replaced by a floxed PGK-neomycin expression cassette. The resistance cassette was removed in vivo by crossing mice to a C57BL/6 Deleter mouse line. Gene disruption was confirmed by RT-PCR and Northern blot analysis. (J:86466)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Bbc3^tm1Ast/Bbc3^tm1Ast	C57BL/6-Bbc3^tm1Ast
hm2	Bbc3^tm1Ast/Bbc3^tm1Ast	involves: C57BL/6 * NZB
cx3	Bbc3^tm1Ast/Bbc3^tm1Ast Tg(SOD1*G93A)1Gur/0	involves: C57BL/6 * NZB * SJL

Phenotypes:

Affected Systems	hm1	hm2	cx3
show or hide all annotated terms
behavior/neurological			√
behavior/neurological phenotype			N
decreased grip strength			√
cellular	√
decreased sensitivity to induced cell death	√
decreased cellular sensitivity to gamma-irradiation	√
growth/size/body			√
weight loss			√
hematopoietic system		√
increased leukocyte cell number		√
increased CD8-positive, alpha-beta T cell number		√
abnormal splenic cell ratio		√
abnormal CD8-positive, alpha-beta T cell physiology		√
immune system		√
immune system phenotype		N
increased leukocyte cell number		√
increased CD8-positive, alpha-beta T cell number		√
abnormal splenic cell ratio		√
abnormal CD8-positive, alpha-beta T cell physiology		√
mortality/aging			√
premature death			√
nervous system		√	√
gliosis			√
motor neuron degeneration			√
abnormal neuron physiology		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	4 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Bbc3^tm1Ast

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bbc3 Mutation: 19 strains or lines available

References

• Original: J:86466 Villunger A, et al., p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science. 2003 Nov 7;302(5647):1036-8
• All: 71 reference(s)