Bbc3^tm1Ast
Targeted Allele Detail

Summary

• Symbol: Bbc3^tm1Ast
• Name: BCL2 binding component 3; targeted mutation 1, Andreas Strasser
• MGI ID: MGI:2681654
• Synonyms: puma^-
• Gene: Bbc3 Location: Chr7:16042318-16052130 bp, + strand Genetic Position: Chr7, 8.85 cM
• Alliance: Bbc3^tm1Ast page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86466
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 which contain the BH3 interaction domain were replaced by a floxed PGK-neomycin expression cassette. The resistance cassette was removed in vivo by crossing mice to a C57BL/6 Deleter mouse line. Gene disruption was confirmed by RT-PCR and Northern blot analysis. (J:86466)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Bbc3^tm1Ast

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bbc3 Mutation: 19 strains or lines available

References

• Original: J:86466 Villunger A, et al., p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science. 2003 Nov 7;302(5647):1036-8
• All: 71 reference(s)