Tg(PLFSADA)2465Rkmb
Transgene Detail

Summary

• Symbol: Tg(PLFSADA)2465Rkmb
• Name: transgene insertion 2465, Michael R Blackburn
• MGI ID: MGI:2681791
• Synonyms: Tg(Ada)2448Mw
• Transgene: Tg(PLFSADA)2465Rkmb Location: unknown
• Alliance: Tg(PLFSADA)2465Rkmb page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(PLFSADA)2465Rkmb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ada (MGI:87916)

• Mutation details: A construct was made that included a 2.8 kb genomic fragment containing the endogenous polyadenylation sequence, intron 11, and ~2kb of 3'-untranslated region and 3' flanking region of the gene. The endogenous promoter was replaced with a promoter containing a 36-bp deletion in the 5'-untranslated region. This resulted in a construct that contained the gene's regulatory elements, allowing expression in the placenta prenatally and the forestomach postnatally, while the 36-bp deletion in the promoter allowed distinction of this transcript from that of native transcript. RNase protection showed transgene transcript in placentas, but not fetuses, of transgenic animals. Enzymatic activity of the protein product was increased in the placenta, but not in the fetus, of transgenic mice compared to nontransgenic animals. (J:29350)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:29350 Blackburn MR, et al., Tissue-specific rescue suggests that placental adenosine deaminase is important for fetal development in mice. J Biol Chem. 1995 Oct 13;270(41):23891-4
• All: 10 reference(s)