Prnp^{tm1(PRNP)Tkit}
Targeted Allele Detail

Summary

• Symbol: Prnp^{tm1(PRNP)Tkit}
• Name: prion protein; targeted mutation 1, Tetsuyuki Kitamoto
• MGI ID: MGI:2681980
• Synonyms: Hu-Ki, Ki-ChM
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^{tm1(PRNP)Tkit} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33412
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Prnp^{tm1(PRNP)Tkit} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PRNP (5621)

  Prnp	(MGI:97769)

• Mutation details: The majority of the endogenous mouse gene was replaced with mutant human PRNP sequence. The human sequence contained a nonsense mutation at codon 145. 3' mouse sequence remained downstream of the inserted human sequence. (J:33412, J:86603)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 142 strains or lines available

References

• Original: J:33412 Kitamoto T, et al., Humanized prion protein knock-in by Cre-induced site-specific recombination in the mouse. Biochem Biophys Res Commun. 1996 May 24;222(3):742-7
• All: 3 reference(s)