Prnp^tm2Rcm
Targeted Allele Detail

Summary

• Symbol: Prnp^tm2Rcm
• Name: prion protein; targeted mutation 2, Richard C Moore
• MGI ID: MGI:2682351
• Synonyms: Rcm0
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^tm2Rcm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:42407
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment encompassing the entire coding region contained within exon 3 as well as approximately 1 kb of sequence 5' to exon 3 was replaced with a PGK-HPRT cassette. The deleted upstream sequence included the exon 3 splice acceptor site. RT-PCR analysis of RNA obtained from homozygous mutant brain tissue showed increased levels of chimeric transcript consisting of Prnp and Prnd sequence relative to wild-type samples. (J:42407)

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 142 strains or lines available

References

• Original: J:42407 Moore RC, et al., Ataxia in prion protein (PrP)-deficient mice is associated with upregulation of the novel PrP-like protein doppel. J Mol Biol. 1999 Oct 1;292(4):797-817
• All: 3 reference(s)