Slc6a5^tm1Betz
Targeted Allele Detail

Summary

• Symbol: Slc6a5^tm1Betz
• Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5; targeted mutation 1, Heinrich Betz
• MGI ID: MGI:2682554
• Synonyms: GlyT2^-/-
• Gene: Slc6a5 Location: Chr7:49559894-49613604 bp, + strand Genetic Position: Chr7, 31.71 cM, cytoband B3
• Alliance: Slc6a5^tm1Betz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86625
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.1 kb genomic fragment encompassing the exons encoding the fourth transmembrane domain was replaced with a neomycin selection cassette inserted by homologous recombination. Neither transcript nor protein was detected by RT-PCR or Western blot analyses of homozygous mutant brain stem tissue. The absence of protein was also verified in fractions obtained from mutant spinal cord extracts. (J:86625)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a5 Mutation: 59 strains or lines available

References

• Original: J:86625 Gomeza J, et al., Deletion of the mouse glycine transporter 2 results in a hyperekplexia phenotype and postnatal lethality. Neuron. 2003 Nov 13;40(4):797-806
• All: 2 reference(s)