H3f3a^{Gt(ROSABetageo)14E7Ev}
Gene trapped Allele Detail

Summary

• Symbol: H3f3a^{Gt(ROSABetageo)14E7Ev}
• Name: H3.3 histone A; gene trap 14E7, Martin Evans
• MGI ID: MGI:2682911
• Synonyms: GTE.14E7
• Gene: H3f3a Location: Chr1:180630125-180641127 bp, - strand Genetic Position: Chr1, 84.46 cM, cytoband D2.3
• Alliance: H3f3a^{Gt(ROSABetageo)14E7Ev} page

Mutation origin

• Mutant Cell Line: GTE.14E7
• Germline Transmission: Earliest citation of germline transmission: J:58812
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: ROSABetageo Vector Type: gene trap
• Mutation details: A retroviral gene trap vector containing a lacZ/neo fusion gene integrated into the first intron of the gene. Although not detectable by Northern analysis, detection of transcript by RT-PCR indicated that the mutation is a hypomorph. (J:58812)

Expression

In Mice Carrying this Mutation:	35 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H3f3a Mutation: 29 strains or lines available

Notes

ES cell line = CCE-1B.

References

• Original: J:58812 Couldrey C, et al., A retroviral gene trap insertion into the histone 3.3A gene causes partial neonatal lethality, stunted growth, neuromuscular deficits and male sub-fertility in transgenic mice. Hum Mol Genet. 1999 Dec;8(13):2489-95
• All: 1 reference(s)