Hbegf^tm2Mek
Targeted Allele Detail

Summary

• Symbol: Hbegf^tm2Mek
• Name: heparin-binding EGF-like growth factor; targeted mutation 2, Eisuke Mekada
• MGI ID: MGI:2682958
• Synonyms: HB^uc
• Gene: Hbegf Location: Chr18:36637980-36648858 bp, - strand Genetic Position: Chr18, 19.46 cM
• Alliance: Hbegf^tm2Mek page

Enlarged heart with fibrosis and poor left ventricular contraction in Hbegf^tm2Mek/Hbegf^tm2Mek mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86547
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Point mutations were introduced in the cDNA sequence which transformed the amino acids residues at positions 148-149 from Leu-Pro to Ser-Thr. The modified cDNA sequence was then fused to the start codon of exon 1 along with IRES, a neomycin resistance gene and a polyadenylation sequence. It is predicted that the resulting product would not be cleavable to release the soluble form of the protein. (J:86547)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hbegf Mutation: 26 strains or lines available

References

• Original: J:86547 Yamazaki S, et al., Mice with defects in HB-EGF ectodomain shedding show severe developmental abnormalities. J Cell Biol. 2003 Nov 10;163(3):469-75
• All: 3 reference(s)