Hbegf^tm3Mek
Targeted Allele Detail

Summary

• Symbol: Hbegf^tm3Mek
• Name: heparin-binding EGF-like growth factor; targeted mutation 3, Eisuke Mekada
• MGI ID: MGI:2682961
• Synonyms: HB^delta-tm
• Gene: Hbegf Location: Chr18:36637980-36648858 bp, - strand Genetic Position: Chr18, 19.46 cM
• Alliance: Hbegf^tm3Mek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86547
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A stop codon was introduced in the cDNA sequence between the codons for amino acid residue 148 and 149. The modified cDNA sequence was then fused to the start codon of exon 1 along with IRES, a neomycin resistance gene and a polyadenylation sequence. RT-PCR of skin and heart specimens confirmed the presence of the modified mRNA transcript. The predicted soluble protein product was detected immunologically. (J:86547)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hbegf Mutation: 26 strains or lines available

References

• Original: J:86547 Yamazaki S, et al., Mice with defects in HB-EGF ectodomain shedding show severe developmental abnormalities. J Cell Biol. 2003 Nov 10;163(3):469-75
• All: 1 reference(s)