Hoxb8^tm1Omm
Targeted Allele Detail

Summary

• Symbol: Hoxb8^tm1Omm
• Name: homeobox B8; targeted mutation 1, Olga Medina-Martinez
• MGI ID: MGI:2683089
• Synonyms: Hoxb8^hp
• Gene: Hoxb8 Location: Chr11:96172731-96176141 bp, + strand Genetic Position: Chr11, 59.82 cM
• Alliance: Hoxb8^tm1Omm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86684
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Missense mutations were incorporated into the LFPWMR motif using a hit and run gene targeting technique. The codons encoding proline and tryptophan were mutated to encode valine and tyrosine, respectively (CCC(P) to GTA(V) and TGG(W) to TAC(Y)). Western blot analysis of fibroblast extracts the level of mutant protein in homozygous cells to be similar to that of normal protein in wild-type cells. (J:86684)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxb8 Mutation: 8 strains or lines available

References

• Original: J:86684 Medina-Martinez O, et al., In vivo mutagenesis of the Hoxb8 hexapeptide domain leads to dominant homeotic transformations that mimic the loss-of-function mutations in genes of the Hoxb cluster. Dev Biol. 2003 Dec 1;264(1):77-90
• All: 1 reference(s)