Pafah1b1^{Gt(IRESBetageo)1Hha}
Gene trapped Allele Detail

Summary

• Symbol: Pafah1b1^{Gt(IRESBetageo)1Hha}
• Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; gene trap 1, Hans-Henning Arnold
• MGI ID: MGI:2683481
• Synonyms: LIS1^GT/GT
• Gene: Pafah1b1 Location: Chr11:74564775-74615210 bp, - strand Genetic Position: Chr11, 45.76 cM, cytoband B3
• Alliance: Pafah1b1^{Gt(IRESBetageo)1Hha} page

Testis abnormalities in Pafah1b1^{Gt(IRESBetageo)1Hha}/Pafah1b1^{Gt(IRESBetageo)1Hha} mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86720
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The expression of a testis-specific transcript was abrogated by the integration a gene trap vector into the endogenous locus. The vector inserted into intron 2 and consisted of an engrailed 2 intron, IRES, and beta-geo. RT-PCR analysis showed the level of a testis-specific transcript, containing exon 2a, to be markedly diminished in homozygous mutant mice. While protein was detected in homozygous mutant brain tissue by Western blot analysis, it was absent in the testes of mutant mice. (J:86720)

Expression

In Mice Carrying this Mutation:	51 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pafah1b1 Mutation: 160 strains or lines available

References

• Original: J:86720 Nayernia K, et al., Inactivation of a testis-specific Lis1 transcript in mice prevents spermatid differentiation and causes male infertility. J Biol Chem. 2003 Nov 28;278(48):48377-85
• All: 2 reference(s)