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Rr27tm2Tilg
Targeted Allele Detail
Summary
Symbol: Rr27tm2Tilg
Name: regulatory region 27; targeted mutation 2, Shirley M Tilghman
MGI ID: MGI:2683633
Synonyms: H19tm4Tilg
Gene: Rr27  Location: unknown  Genetic Position: Chr7, Syntenic
Alliance: Rr27tm2Tilg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:86440
Parent Cell Line:  CJ7 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Modified regulatory region)
Mutation:    Nucleotide substitutions
 
Mutation detailsMutations were introduced at each of the 3 AB sites (AB-1, AB-2, and AB-3) located in the imprinting control region (ICR) upstream of the H19 transcription start site. Single loxP sites remained flanking the ICR after a floxed cassette containing a neomycin resistance gene and a cre recombinase gene was self-excised. The mutations were shown by EMSA to ablate binding activity. Imprint establishment and maintenance was unaffected in mutant mice. (J:86440)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rr27 Mutation:  7 strains or lines available
Notes
This locus is subject to imprinting, however the introduced mutations at the AB sites did not affect the establishment or maintenance of the male-specific epigenetic state in the sperm.
References
Original:  J:86440 Bowman AB, et al., Functional characterization of a testis-specific DNA binding activity at the H19/Igf2 imprinting control region. Mol Cell Biol. 2003 Nov;23(22):8345-51
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory