Rr27^tm2Tilg
Targeted Allele Detail

Summary

• Symbol: Rr27^tm2Tilg
• Name: regulatory region 27; targeted mutation 2, Shirley M Tilghman
• MGI ID: MGI:2683633
• Synonyms: H19^tm4Tilg
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm2Tilg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86440
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Nucleotide substitutions
• Mutation details: Mutations were introduced at each of the 3 AB sites (AB-1, AB-2, and AB-3) located in the imprinting control region (ICR) upstream of the H19 transcription start site. Single loxP sites remained flanking the ICR after a floxed cassette containing a neomycin resistance gene and a cre recombinase gene was self-excised. The mutations were shown by EMSA to ablate binding activity. Imprint establishment and maintenance was unaffected in mutant mice. (J:86440)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

Notes

This locus is subject to imprinting, however the introduced mutations at the AB sites did not affect the establishment or maintenance of the male-specific epigenetic state in the sperm.

References

• Original: J:86440 Bowman AB, et al., Functional characterization of a testis-specific DNA binding activity at the H19/Igf2 imprinting control region. Mol Cell Biol. 2003 Nov;23(22):8345-51
• All: 1 reference(s)