St14^tm1Bug
Targeted Allele Detail

Summary

• Symbol: St14^tm1Bug
• Name: suppression of tumorigenicity 14 (colon carcinoma); targeted mutation 1, Thomas H Bugge
• MGI ID: MGI:2683707
• Synonyms: Matriptase/MT-SP1
• Gene: St14 Location: Chr9:31000698-31043149 bp, - strand Genetic Position: Chr9, 16.61 cM
• Alliance: St14^tm1Bug page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87125
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment encompassing 350 bp of the putative intron 1 and the majority of the putative exon 2 was replaced with an hprt cassette inserted by homologous recombination. The deleted region encoded 19 of the 24 residues of the signal anchor required for membrane translocation. RT-PCR analysis identified mutant transcript in which the putative exon 1 spliced to the putative exon 3. This aberrant splice event results in a frameshift and ultimately a premature stop codon. (J:87125)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any St14 Mutation: 45 strains or lines available

References

• Original: J:87125 List K, et al., Matriptase/MT-SP1 is required for postnatal survival, epidermal barrier function, hair follicle development, and thymic homeostasis. Oncogene. 2002 May 23;21(23):3765-79
• All: 15 reference(s)