Omp^tm2(spH)Mom
Targeted Allele Detail

Summary

• Symbol: Omp^tm2(spH)Mom
• Name: olfactory marker protein; targeted mutation 2, Peter Mombaerts
• MGI ID: MGI:2684190
• Synonyms: OMP-spH, OMP-synapto-pHluorin
• Gene: Omp Location: Chr7:97792566-97794654 bp, - strand Genetic Position: Chr7, 53.59 cM
• Alliance: Omp^tm2(spH)Mom page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88765
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding exon was replaced by a construct containing the ecliptic synaptopHluorin (spH) coding sequence and a self-excising neomycin resistance cassette. SpH is a fusion protein joining a pH-sensitive derivative of wild-type green fluorescent protein (GFP) to the carboxyl terminus of a mouse synaptic vesicle-associated membrane protein (VAMP2), which targets spH to the lumenal face of pre-synaptic vesicles. The loxP-flanked, male-germline self-excising neomycin resistance cassette (which was deleted in the chimeric founder's germline) contains the Cre recombinase coding sequence under control of the mouse testis-specific angiotensin-converting enzyme (tACE) promoter and tk-neo. The endogenous gene regulatory sequences direct spH/Vamp expression specifically to sensory neurons in glomeruli of the olfactory bulb. (J:36595, J:79620, J:88765)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Omp Mutation: 27 strains or lines available

Notes

In the acidic lumen of pre-synaptic vesicles, the fluorescence of spH is only about 5% of its intensity at neutral pH. When synaptic vesicles with spH anchored to their lumenal face fuse with the plasma membrane to release their contents, the spH is exposed to the pH-neutral extracellular environmment and rapidly becomes highly fluorescent. All olfactory bulb glomeruli fluoresce, with varying intensity. The superficial nerve layer overlying the glomerular layer and comprising axons of olfactory sensory neurons is more weakly fluorescent. In vivo fluorescent imaging of the olfactory bulb demonstrates that odorant-evoked responses cause strong fluorescence of odorant-specific, bilaterally symmetric groups of glomeruli.

References

• Original: J:88765 Bozza T, et al., In vivo imaging of neuronal activity by targeted expression of a genetically encoded probe in the mouse. Neuron. 2004 Apr 8;42(1):9-21
• All: 19 reference(s)