Gt(ROSA)26Sor^{tm1(Notch1)Dam}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Notch1)Dam}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Douglas A Melton
• MGI ID: MGI:2684314
• Synonyms: Lox-stop-Lox-Rosa^{NICD-ires-GFP}, R26^fN1-ICD, R26fsN1ICD, R26N1ICD, R26-NICD, R26^NotchIC, R-NICD, Rosa26^LSL-NICD, Rosa26^N1ICD, Rosa26^NIC, Rosa26^{NotchIC-IRES-GFP}, Rosa26-Stop^Floxed-NICD, RosaICN^LSL, Rosa^N1-IC, Rosa^NICD, ROSA-NICD, Rosa^NICD1, Rosa^NICDflox, Rosa^Notch, Rosa-NotchIC-IRES-GFP, TG^NICDflox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Notch1)Dam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86975
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Notch1)Dam} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Notch1 (MGI:97363)

• Mutation details: A targeting vector containing a floxed neo cassette followed by a mouse Notch1 sequence fragment and GFP was inserted at the endogenous locus. Excision of the floxed neo cassette leads to the expression of the Notch1 sequence encoding an intracellular portion of NOTCH1 (amino acids 1749-2293), but lacking the c-terminal PEST domain. IRES preceded the sequence encoding the nuclear-localized enhanced GFP. (J:86975)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	16 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm1(Notch1)Dam}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Liposarcoma, Dedifferentiated J:237232 in double Gt(ROSA)26Sor^{tm1(Notch1)Dam} and Tg(Adipoq-cre)1Evdr mutants.

Phenotypic Similarity to Human Syndrome: Osteosclerosis J: 233131 in double Gt(ROSA)26Sor^{tm1(Notch1)Dam} and Tg(Col1a1-cre)1Kry mutants.

References

• Original: J:86975 Murtaugh LC, et al., Notch signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14920-5
• All: 237 reference(s)