Rac1^tm1Tyb
Targeted Allele Detail

Summary

• Symbol: Rac1^tm1Tyb
• Name: Rac family small GTPase 1; targeted mutation 1, Victor L J Tybulewicz
• MGI ID: MGI:2684419
• Synonyms: Rac1^flox
• Gene: Rac1 Location: Chr5:143491236-143513786 bp, - strand Genetic Position: Chr5, 82.22 cM
• Alliance: Rac1^tm1Tyb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86765
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Targeting took place in ES cells homozyogus for the cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase only in haploid spermatids. A loxP site was introduced between exons 3 and 4 while a floxed PGK-neomycin resistance gene was introduced in reverse orientation between exons 5 and 6. Cre recombination in the male germline removed the neomycin gene, leaving exons 4 and 5 flanked by loxP sites. (J:86765)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Rac1^tm1Tyb

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rac1 Mutation: 24 strains or lines available

References

• Original: J:86765 Walmsley MJ, et al., Critical roles for Rac1 and Rac2 GTPases in B cell development and signaling. Science. 2003 Oct 17;302(5644):459-62
• All: 42 reference(s)