Hcn4^tm1Jsr
Targeted Allele Detail

Summary

• Symbol: Hcn4^tm1Jsr
• Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4; targeted mutation 1, Juliane Stieber
• MGI ID: MGI:2684537
• Gene: Hcn4 Location: Chr9:58730695-58770458 bp, + strand Genetic Position: Chr9, 31.8 cM, cytoband C
• Alliance: Hcn4^tm1Jsr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86988
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neomycin resistance gene was inserted in intron 3 and an additional loxP site was inserted in intron 4. Transient transfection of properly targeted ES cells with a Cre expressing plasmid resulted in excision of the neomycin gene leaving exon 4 flanked by two loxP sites. (J:86988)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hcn4 Mutation: 67 strains or lines available

References

• Original: J:86988 Stieber J, et al., The hyperpolarization-activated channel HCN4 is required for the generation of pacemaker action potentials in the embryonic heart. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15235-40
• All: 6 reference(s)