Casp3^tm1Kin
Targeted Allele Detail

Summary

• Symbol: Casp3^tm1Kin
• Name: caspase 3; targeted mutation 1, Ken-Ichi Nakayama
• MGI ID: MGI:2684608
• Synonyms: caspase-3 -
• Gene: Casp3 Location: Chr8:47070326-47092724 bp, + strand Genetic Position: Chr8, 26.39 cM
• Alliance: Casp3^tm1Kin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69809
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a 9.8 kb genomic fragment containing sequences encoding the QACRG motif of the catalytic site of the encoded protien. RT-PCR and immunohistochemistry experiments confirmed that no detectable transcript and protein was made from this allele. (J:69809)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp3 Mutation: 33 strains or lines available

References

• Original: J:69809 Morishita H, et al., Deafness due to degeneration of cochlear neurons in caspase-3-deficient mice. Biochem Biophys Res Commun. 2001 Jun 1;284(1):142-9
• All: 1 reference(s)