Fgf8^tm3Mrc
Targeted Allele Detail

Summary

• Symbol: Fgf8^tm3Mrc
• Name: fibroblast growth factor 8; targeted mutation 3, Mario R Capecchi
• MGI ID: MGI:2686826
• Synonyms: Fgf8^APN, Fgf8^CR, Fgf8^H, Fgf8^hy
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^tm3Mrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66265
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: This hypomorphic allele was generated by inserting an frt-flanked neomycin cassette into intron 5. Single loxP sites located in introns 4 and 5, flank exon 5 and the neomycin cassette. An alkaline phosphatase gene was inserted just 5' to the second lox P site such that upon cre-mediated recombination, the alkaline phosphatase gene is expressed under the control of the Fgf8 gene. Fgf8^tm1Mrc and Fgf8^tm2Mrc were respectively generated by cre- and flp-mediated recombination of this allele. (J:66265, J:87304)

Expression

In Mice Carrying this Mutation:	15 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 21 strains or lines available

References

• Original: J:66265 Moon AM, et al., Fgf8 is required for outgrowth and patterning of the limbs. Nat Genet. 2000 Dec;26(4):455-9
• All: 8 reference(s)