Hcn1^tm2Kndl
Targeted Allele Detail

Summary

• Symbol: Hcn1^tm2Kndl
• Name: hyperpolarization activated cyclic nucleotide gated potassium channel 1; targeted mutation 2, Eric R Kandel
• MGI ID: MGI:2686951
• Synonyms: Hcn1-, HCN1 knockout
• Gene: Hcn1 Location: Chr13:117738856-118117564 bp, + strand Genetic Position: Chr13, 66.34 cM
• Alliance: Hcn1^tm2Kndl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86763
• Parent Cell Line: MM13 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of the pore-S6 domain containing exon. At the same time, a floxed neomycin cassette was introduced downstream. Cells in which transient Cre recombinase expression of properly targeted ES cells resulted in excision of both the neomycin gene and the pore-S6 exon were selected. Both mRNA and protein were shown to be absent in the brains of mice homozygous for this allele. (J:86763)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Hcn1 Mutation: 49 strains or lines available

References

• Original: J:86763 Nolan MF, et al., The hyperpolarization-activated HCN1 channel is important for motor learning and neuronal integration by cerebellar Purkinje cells. Cell. 2003 Nov 26;115(5):551-64
• All: 39 reference(s)