Nefm^tm1Mvr
Targeted Allele Detail

Summary

• Symbol: Nefm^tm1Mvr
• Name: neurofilament, medium polypeptide; targeted mutation 1, Mala V Rao
• MGI ID: MGI:2686958
• Synonyms: NF-M^taildelta
• Gene: Nefm Location: Chr14:68356994-68362453 bp, - strand Genetic Position: Chr14, 35.46 cM
• Alliance: Nefm^tm1Mvr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87157
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding 426 C-terminal residues that make up the carboxyl tail domain was replaced with a myc epitope tag and a neomycin selection cassette. The disruption was designed to leave neurofilament subunit stoichiometry unaltered. Truncated protein was detected in mutant mice by Western blot analysis of sciatic and optic nerve extracts. Normal protein was not revealed in homozygous mutant mice by these tests. (J:87157)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nefm Mutation: 28 strains or lines available

References

• Original: J:87157 Rao MV, et al., The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. J Cell Biol. 2003 Dec 8;163(5):1021-31
• All: 5 reference(s)