Pax3^tm1Buck
Targeted Allele Detail

Summary

• Symbol: Pax3^tm1Buck
• Name: paired box 3; targeted mutation 1, Margaret E Buckingham
• MGI ID: MGI:2687297
• Synonyms: P34TKZ Pax3 reporter, Pax3^-, Pax3^ILZ, Pax3^IRESnLacZ, Pax3^nLacZ
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^tm1Buck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86911
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 3' portion of exon 2 was replaced with a cassette containing IRES-lacZ followed by a polyadenylation signal. The targeting vector contained a floxed puromycin resistance gene, however this was excised via cre mediated recombination, leaving only a single loxP site following the lacZ cassette. The expression pattern of lacZ was shown to be similar to that of the endogenous gene. (J:86911)

Expression

In Mice Carrying this Mutation:	121 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

References

• Original: J:86911 Relaix F, et al., The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo. Genes Dev. 2003 Dec 1;17(23):2950-65
• All: 24 reference(s)